IASH Community Forum : Sampling and testing for microbial contamination.
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 Subject : PROPOSED NEW ASTM STD PRACTICE - FUEL QPCR.. 07/01/2021 01:14:18 PM 
Dr. Frederick Passman
Posts: 2
Location: BCA, Inc. Princeton NJ USA
ASTM D02.14 has recently balloted Standard Practice for Quantification of Microbial Contamination in Liquid Fuels and Fuel-Associated Water by Quantitative Polymerase Chain Reaction (qPCR). The proposed new standard is now in the queue for D02 main committee ballot.

Thanks to Oscar Ruiz for his great work as the proposed new standard's Technical Contact and primary author, and to the Work Item 65790 task force members who have contributed their suggested revisions and have reviewed/commented to numerous iterations of the draft standard!

Cheers,
Fred
Fred recently celebrated 50 years as a practicing microbial ecologist. He has been investigating fuel microbiology issues only since 1982.
 Subject : Re:PROPOSED NEW ASTM STD PRACTICE - FUEL QPCR.. 12/06/2021 11:09:49 AM 
Mr. Graham Hill
Posts: 1
Location: Cardiff, Wales, UK
Great work Oscar, Fred and all. Next step the sampling standard review! As we all know the test is only as good as the sample!
29 years experience in microbiological contamination and Microbially Influenced Corrosion issues in petroleum, aviation, energy, marine, transportations sector. Testing (Lab and Field Kits), Consultancy, Training and Research solutions.
 Subject : Re:PROPOSED NEW ASTM STD PRACTICE - FUEL QPCR.. 12/06/2021 11:18:00 AM 
Dr. Steven Zabarnick
Posts: 2
Location
I believe the D02 ballot has also now passed and the spec should be published soon.
 Subject : Re:PROPOSED NEW ASTM STD PRACTICE - FUEL QPCR.. 12/28/2021 02:21:35 PM 
Dr. Frederick Passman
Posts: 2
Location: BCA, Inc. Princeton NJ USA
After some wrangling over whether the standard was to be a practice, test method, or guide, it was balloted and approved as ASTM D8142 Standard Guide for Quantification of Microbial Contamination in Liquid Fuels and Fuel-Associated Water by Quantitative Polymerase Chain Reaction (qPCR).

It wasn't a Test Method because one or more protocol steps offered multiple options.

It wasn't a Practice because qPCR generates a result.

With the Guide now approved, the ASTM D02.14 Fuel Microbiology WG has several potential paths forward.
1. Develop a Practice for extracting and purifying DNA from test specimens.
2. Develop target-specific qPCR-based Test Methods.
3. Assess whether Practice D7464 needs language specific to sample collection and handling when genomic testing is to be performed.

WK78425 has been registered for the D7464 revision effort.
We are now waiting for volunteers willing to champion new ASTM standards.
Fred recently celebrated 50 years as a practicing microbial ecologist. He has been investigating fuel microbiology issues only since 1982.
 
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